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TB-500 (Thymosin Beta-4 Fragment) TB-500 is a synthetic fragment of Thymosin Beta-4, a naturally occurring peptide involved in actin regulation and cellular repair

Reconstitution Add 3.0ml (300 units on an insulin syringe) of bacteriostatic water to the 70mg vial

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Cells were cultured in glass bottom dishes (ibidi, Grfelfing), and multipoint images over 10 XY fields were acquired every 1 min using a 60 Plan Apo 1.4 numerical aperture (NA) Nikon oil objective (Nikon) and Perfect Focus 2.0 engaged on the CSU W1 Confocal Scanner Unit (Yokogawa) attached to a Nikon ECLIPSE Ti2 microscope with a Photometrics Prime 95B camera (Teledyne Photometrics)