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Mouse islets were subsequently washed, purified using a Histopaque gradient (Histopaque-1119 and Histopaque-1083, SigmaAldrich) and rested overnight at 37 C in a 95% O 2 /5% CO 2 incubator before performing experiments in mouse islet culture medium (RPMI-1640 with 11 mM D-glucose, supplemented with 10% FBS and 1% penicillin/streptomycin)

Rotation protocol Rotate injection sites every week

When significant weight loss occursas commonly seen with tirzepatide therapymultiple physiological changes may affect temperature regulation