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Additionally, iron has an important role in the development of oligodendrocytes, the cells in the brain that produce myelin (19)

At the same concentration of 580 M, Cu(OAc) 2 induced a higher upregulation of IL-1 than CuCl 2 , while CuCl 2 induced a higher upregulation of IL-8, HSPA1A and FOSL1 than Cu(OAc) 2

Furthermore, trypsin mRNA expression as a marker for acinar cell contamination was assessed using PCR (primers: Table 1, PCR conditions: Tm=60 C, cycles 40)

Animals are tested in glass cylinder, where they adapt for 10 minutes prior to assessment

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