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Relative expression levels were determined by real-time PCR on a Bio-Rad CFX Connect or Real-Time System (CFX96 Real-Time System) using SYBR Green or the PowerUp SYBR Green kit (Thermo Fisher Scientific), and the primer sequences are listed in Supplemental Methods

10.1161/CIRCRESAHA.114.301958 161

Injectables, inevitably, are honing their marketing too: Galderma, maker of Sculptra and Restylane, introduced its SCULPT & LIFT protocol, updating injectable guidance after a study on medication-driven weight loss

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