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Low levels of artificial light at night (5 lux) disrupt circadian rhythmicity with a reduction in Per1 and Per2 expression in the SCN, concurrent with altered expression of Bmal1, Per1, Per2 , and Cry1 in the liver of mice (Fonken et al., 2013)
Using 3.0mL of bacteriostatic water creates a concentration of approximately 26.7mg/mL
Periodic reassessment every 6 to 12 weeks by labs and outcome measures enables clinicians to fine-tune the protocol
These dates usually show best-by recommendations when the water tastes best rather than true expiration dates
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