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48 GSH levels were determined within the volume of interest using MEGA-PRESS double-editing for the cysteinyl -CH 2 residue of GSH 7 (repetition time=2,000 ms, echo time=122 ms, free induction decay points=2,048, spectral width=2,000 Hz, number of averages=8 per phase cycle ON or OFF, 320 acquisitions total requiring just under 11 min)

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This result is consistent with previous studies that reported cardiac succinate accumulation during ischemia 53 , but is the first demonstration of ischemic succinate in the pericardial fluid

Consequently, GPX4 inhibitors, including RSL3 42 and the chloroacetamides described here display senolytic activity

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