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Acknowledgements This work was partially supported by the Research Council of Norway through its Centers of Excellence scheme, project number 332727, the Global Health and vaccination research (GLOBVAC) program, project 285136 (J.T.A., S.F., D.S., J.B., S.A.S.), the grants 274993, 287927 (J.T.A
Cell numbers were determined by plating for colony counts
For peptides that do not dissolve cleanly in bacteriostatic water alone typically those with hydrophobic sequences or basic amino acid-rich compositions that require protonation for solubilisation the recommended approach is to first perform initial reconstitution in a small volume of 0.1% acetic acid water (also available from Peptides Lab UK) to produce a clear stock solution, before diluting further with bacteriostatic water or the relevant physiological buffer
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