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The entire explant is imaged for all antibody and nuclear labeling in a 3-dimensional X-Y-Z plane in which the Z-plane consists of a stack imaged at intervals of 0.5-5 m

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During development and progression of atherosclerosis, Nrf2 signaling modulates many physiological and pathophysiological processes, like regulation of lipid homeostasis, CD36 gene expression regulation, foam cell formation, macrophage polarization, immunity regulation (Th2 differentiation and inhibition of pro-inflammatory gene expression through NFB down-regulation), redox regulation and inflammation, improvement of endothelial dysfunction, as well as GSH synthesis and utilization (234245)