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Glutathione therapy should be administered by specialists under medical supervision

doi: 10.1038/nrendo.2013.251

Standard confocal fluorescence microscopy Cells were seeded on round glass coverslips (Fisherbrand) and treated according to each individual experimental setup

A total of 30 male BALB/c nude mice were randomly divided into four groups: a CT26 + 0.9%NaCl group (8 mice bearing CT26 tumors, treated with vehicle, as a control model of cancer cachexia), a 0.9%NaCl group (6 mice without tumors, treated with the vehicle, as normal controls), a CT26 + LC1 group (8 mice with CT26 tumors, treated with1 mg/kg bodyweight L-carnitine) and a CT26 + LC10 group (8 mice with CT26 tumors treated with10 mg/kg bodyweight L-carnitine)

and (ii) induces a quantitative rise of Nrf2 and glutathione that could only be achieved by the highest non-toxic concentrations of the potent experimental Nrf2-activator DEM