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The second antibody was a biotinylated goat anti-rabbit immunoglobulin, and the signal was detected using the Vectastain ABC kit (Vector Laboratories, Burlingame, California, USA) with DAB as a substrate

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Syndecans consist of conserved cytoplasmic and transmembrane domains and more divergent ectodomains that carry differentially sulfated glycosaminoglycan (GAG) chains 2