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Studies have shown that glucose concentrations above 25 mM trigger ferroptosis, marked by increased levels of malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), and Fe + , as well as altered expression of ACSL4, ALOX15, GPX4, and SLC7A11 (208, 209)

Giemsa staining of frozen-thawed spermatozoa (repeated in triplicated) was performed for the acrosome integrity assay (36)