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118 In a double-blind, placebo-controlled trial of 60 mechanically ventilated patients at high risk of developing ventilator-associated pneumonia (VAP), NAC significantly reduced the risk of VAP versus placebo, 26.6% versus 46.6%, and shortened intensive care unit (ICU) stay (14.4 versus 17.8 days) and hospital stay (19.2 versus 24.6 days)

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24 h later, culture media were replaced with 1 mL of serum-free DMEM containing 50 ng/mL of recombinant hTNF- (Peprotech) and cultured for a further 24 h before collecting cells and supernatants

Pericytes are required for bloodbrain barrier integrity during embryogenesis

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