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The FDA-approved titration schedule starts at a low dose (0.25 mg weekly for Ozempic or Wegovy) to minimize gastrointestinal side effects, gradually increasing every four weeks

The two-hour window is a conservative, low-risk approach adopted from the broader drug-interaction guidance in the dulaglutide prescribing information

A secondary antibody solution (PT66, AD0040, PerkinElmer) at a concentration of 0.3 g/mL was added and incubated for 1 h, followed by another five washes with DELFIA wash solution