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doi: 10.3389/fcell.2022.976953 Received 23 June 2022 Accepted 18 July 2022 Published 10 August 2022 Volume 10 - 2022 Edited by Xinjian Zhu, Southeast University, China Reviewed by Nyzil Massey, Iowa State University, United States Manikandan Samidurai, Iowa State University, United States Updates Copyright 2022 Fabisiak and Patel

By 12 months, vitamin D deficiency had reached 13.6%

Histology Euthanized fish were slit open from the anal vent to the gills, and the entire body was fixed with 4% paraformaldehyde in 0.1 M phosphate buffer (Muto, Tokyo, Japan)

20 units = 0.2mL 10mg/mL = 2mg per injection

Your doctor may hold a dose level for additional weeks if side effects are significant, or may accelerate if you are tolerating each stage well