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The Infectious Diseases Society of America recommends amoxicillin-clavulanate rather than amoxicillin as first line treatment and doxycycline, levofloxacin, and moxifloxacin in patients allergic to penicillin.17 The emergence of bacteria resistant to current antibiotic therapies is a worldwide clinical threat

Cell growth and induction of protein expression One single colony of each of the four transformed clones was transferred to a 10 ml LB liquid medium containing 100 mg/L of ampicillin and grown overnight at 37