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Both are frequently used together in preclinical research

hNF-1 was grown and maintained as monolayer cultures at 37 C in 5% CO 2 in a humidified atmosphere, using DMEM (low glucose with l-glutamine and phenol red, Sigma-Aldrich Co., Llc.) and 5 vol% FBS (Thermo Fisher Scientific Inc.)

When the needle is withdrawn, the reservoir cover reseals itself

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