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SIRT1 is a direct coactivator of thyroid hormone receptor 1 with gene-specific actions

2.8 Western blotting Proteins were extracted from the tissue samples, C2C12 myocytes, or HUVECs using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Nacalai Tesque, Kyoto, Japan), separated using SDS-PAGE, and transferred to PVDF membranes

Importantly, the good data don't show these drugs shutting the system down

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