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To aliquot safely: Work in a clean environment, ideally with minimal air movement Use sterile technique throughout Prepare labeled sterile vials or containers in advance Draw from the master vial and dispense into individual containers Refrigerate all aliquots immediately The trade-off is that aliquoting introduces more handling steps where contamination can occur

PMID: 21392607 Coetzer KC, Zllner E, Moosa S

Stamper, B

Am J Clin Nutr 104(3):694703 Arnal MA, Mosoni L, Boirie Y, Houlier ML, Morin L, Verdier E et al (1999) Protein pulse feeding improves protein retention in elderly women

Red and near-infrared LED is non-thermal, so unlike RF or ultrasound it adds no heat to skin that is still settling, and the 24-hour wait simply lets the initial inflammatory phase pass first