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Cell viability was assessed using a Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) according to the manufacturers instructions, with absorbance measured at 450 nm

doi: 10.1038/nature05859 77 YangWSSriRamaratnamRWelschMEShimadaKSkoutaRViswanathanVSet al

6.5 Fecal microbiota transplantation To balances the recipients unbalanced gut microbiota, FMT, which first emerged in China in the 4th century, entails injecting a healthy donors fecal solution into the recipients intestine (256)

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For longer trips, pellet-based coolers (FRIO wallets) use evaporative cooling and function for 57 days when kept damp