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Cell culture HepaRG cells (Invitrogen, California, US) were cultured in growth medium (Williams E medium with Glutamax I, supplemented with 100 U/ml penicillin-streptomycin, 5 g/ml human recombinant insulin and 0.5 M hydrocortisone hemisuccinate) at 37 C with 5% CO2

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Long-chain fatty acyl-CoA thioesters cannot enter the mitochondrial matrix in animals because they are not able to pass through the inner mitochondrial membrane