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Non-esterified short-chain fatty acid (SCFA) quantification After finding that nonesterified short-chain fatty acids (SCFAs) were not detected by our first approach, we measured acetic, propanoic, isobutanoic (isobutyric), butanoic (butyric), isopentanoic (isovaleric), pentanoic (valeric), hexanoic (caproic) and heptanoic acids with a Clarus 500 chromatography system connected to a SQ8s mass spectrometer (Perkin Elmer) using the method described by Togo et al 50

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Double-check, then triple-check your calculations for concentration