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A 20 L aliquot of the supernatant was analyzed using an AB SCIEX QTRAP 5500 LC/triple quadrupole mass spectrometer (Applied Biosystems SCIEX, Foster City, CA, USA)

As expected by the increased striatal DA, pathways signifying activation of signalling by G-protein-coupled receptors, transmission across chemical synapses and calcium signalling were all positively enriched at 3 months in iSPN + mut (Supplementary Table 1a), whereas genes associated with metabolic pathways such as oxidative phosphorylation (OxPhos) and respiratory electron transport ATP synthesis were significantly downregulated in iSPN + mut compared to iSPN+wt at 3 months (Fig