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Purified complex (3 L, ~4 mg/mL) was applied to the grids that were subsequently vitrified in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific) under 100% humidity at 4 C

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The 50 and 130 kDa low-molecular-weight forms specifically produced significant wrinkle-depth reduction by mean roughness (Ra) and maximum roughness (Rz) measurement, attributed to deeper dermal penetration of the smaller molecules [7]

The kidney warnings reflect the potential for indirect effects through volume depletion in susceptible individuals rather than direct nephrotoxicity

These include: A missed period or a much lighter period than usual Nausea, especially in the morning Breast tenderness Fatigue or feeling more tired than normal Frequent urination Spotting or bleeding between periods when it is not expected These signs do not always mean someone is pregnant