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It was centrifuged at 4000 rpm at 4 C for 5 min, 50 l homogenate (supernatant) mixed with 50 l low melting point agarose 1.5% (quick step),0.8% low melting point agarose was taken and cast on the slide add cover slip and then remove the coverslip after drying the gel (approx.10 min)

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You may feel slight pressure or a mild pinch during the injections

Specifically, experimental studies have demonstrated that vancomycin administration in mice exacerbates allergic airway disease symptoms by altering the composition and abundance of gut microbiota, leading to reduced SCFA production