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Additionally, advanced techniques like enzymatic ligation (transglutaminase) and click chemistry are increasingly employed to create highly uniform and stable ADCs (Figure 13)

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GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae

A major defect in cross-regulation involves defective function of regulatory T cells (Treg), often associated with gene variants such as FOXP3 and IL2RA that prevent suppression of this autoimmune cascade [59,60,61,62]

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