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2-5d and 7d give the density of electrostatic potential on the molecular surfaces of NAP and its metabolites DNAP, NAP-AGLU, NAP-GLY, NAP-SU and DNAP-AGLU where red indicates electron-rich regions, green indicates neutral and blue indicates electron-deficient regions

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Bulk RNA-Seq data for 449 COAD samples were retrieved from the TCGA-COAD cohort ( while clinical and pathological information was obtained from UCSC Xena ( In this study, samples lacking complete clinical pathological data or with survival times of less than 1 month were excluded

However, under certain pathological conditions, excessive changes in organ weight due to organ congestion and oedema, hyperplasia and hypertrophy, atrophy or degeneration will occur

A clear band at approximately 30% sucrose concentration was observed, extracted and the presence of EV-F3 was confirmed by SDS-Page gel electrophoresis