The cover slip was slowly removed and the slides were lysed in a solution containing 100 mM EDTA, 2.5 M NaCl, 0.2 M NaOH, and 10 mM Tris (pH 10) with freshly prepared 1% Triton x-100 for 40 min
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58, 59 In contrast, the possibility of GLP-1R expression in cells is controversial, with some reporting it is present, 47, 48 whereas others report no GLP-1R in cells, similar to our results
Beneficial Clostridium may exert beneficial effects on the core pathogenesis of PBC
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