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Quantification of 5-HT, related metabolites, and ABA The quantification of tryptophan, tryptamine, 5-HT, and MT in rice seedlings, as well as 5-HT and MT in cooked rice and ABA in brown rice, were conducted using liquid chromatography-tandem mass spectrometry (LC-MS/MS) at the Wuhan ProNets Testing Technology Co., Ltd