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To increase the number of particles in the holes, grids were re-incubated with a further 34 l of sample for 30 s, followed by mechanical blotting for 34 s and rapid vitrification in a liquid ethane/propane mixture with a Vitrobot Mark IV plunge-freezing device (Thermo Fisher Scientific) operated at 4 C and 100 % relative humidity

As women age, particularly after age 35, the likelihood of aneuploidy in eggs rises

The volume of each nutrient solution administered by gavage was 2 mL