Because real skin change doesnt happen instantly it happens with patience, science, and consistency
Mouse Tumor Cell Isolation, Fluorescence-Activated Cell Sorting and RNA Sequencing p48-Cre/p53F/+KrasL/+/ROSA26-LSL-YFP ( Sirt6 cKO), as well as Sirt6 WT p48-Cre/p53F/+KrasL/+/ROSA26-LSL-YFP as well as 4T1-eGFP tumor bearing mice were euthanized and entire tissues (primary tumors, livers and lungs) were minced and incubated with 1.3mg/ml collagenase (Sigma-Aldrich, C6079) on a horizontal shaker at 37 C for 30 min, vortexing every 5 min
Avoid intense workouts, saunas, or excessive heat for 24 hours
Treatment options, dosing, and eligibility should be discussed with a licensed healthcare professional
In other words, the schedule is a sensible default that a clinician can adjust, not a fixed rule you should try to bend on your own