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g , Relative cell number of the indicated Jurkat SLC25A39 knockout cells expressing a vector control or SLC25A39 cDNA transduced with the indicated sgRNA

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Reverse dimerization assay HeLa cells were co-transfected with the Golgi marker GalT-mRFP (not displayed in all figures) and Go-Nt 7 -FM 4 -GFP, MGNC-Nt 7 -FM 4 -GFP or GFP-FM 4 -hGH (at a 1:3 plasmid ratio), and seeded on poly-L-lysine-coated coverslips for 15 h as indicated above

Ipamorelin is a GHRP that works through ghrelin receptors