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Skewed X chromosome inactivation and female preponderance in autoimmune thyroid disease: an association study and meta-analysis

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Western blotting The total protein was extracted from HK-2 cells treated with methylglyoxal, hydrogen peroxide, and LPS using RIPA lysis buffer (Beyotime, China) supplemented with 1% phenylmethylsulfonyl fluoride (Beyotime, China)