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Liver and kidney function tests can identify any unexpected impacts on organ function

Following permeabilization (0.2% Triton-X100 in PBS, 10 min, RT), endogenous peroxidases were blocked by a 10 min incubation in a 3% H 2 O 2 solution, followed by a 1 h RT incubation in PBS with 2% bovine serum albumin (BSA, Amresco, Solon, OH)

The UPS selectively degrades misfolded or damaged soluble proteins, whereas macroautophagy and selective autophagy pathways eliminate protein aggregates and dysfunctional organelles via lysosomal degradation

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10.1186/s13395-017-0123-0 [DOI] [PMC free article] [PubMed] [Google Scholar] Kaufmann P., Thompson J