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Quantitative real-time polymerase chain reaction (qRT-PCR) for target gene expression was performed using the SensiFAST SYBR No-ROX Kit (Bioline)

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attached proteolysis-targeting chimeras (PROTACs) to lactam cyclic peptide as ER-binding ligand, 6-aminocaproic acid as a linker, and a hydroxylated structure for recruiting E3 ligase known as 16 compound

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And for those tracking overall treatment costs across different approaches, the peptide cost calculator provides useful estimates