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Buffer G of the supernatant was subsequently replaced with 50 mM Tris-HCl (pH 8.0) by using the Sephadex column PD Mini Trap G-25 (GE Healthcare, Uppsala, Sweden), according to the manufacturers protocol

Disturbed cingulate glutamate metabolism in adults with high-functioning autism spectrum disorder: Evidence in support of the excitatory/inhibitory imbalance hypothesis

In addition, experimental animal research has shown that sulforaphane may prevent the development of MetS hallmarks such as diabetes, hypertension, and hyperlipidemia (256)

Ascioti AJ, Hofstetter WL , Miller MJ, Rice DC, Swisher SG, Vaporciyan AA, Roth JA, Putnam JB, Smythe WR, Feig BW, Mansfield PF, Pisters PW, Torres MT, Walsh GL

Open questions heading into 2025 and beyond Important gaps remain