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The culture was grown overnight at 18 C and pelleted at 3000 rpm for 10 min and either proceeded fresh to lysis or stored at 80 C

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I built this matrix by mapping each row to the single trial endpoint that produced the highest-quality primary evidence as of mid-2026: percent body-weight change for efficacy (Jastreboff 2023, SURMOUNT-1, STEP 1), three-point MACE for cardiovascular (SELECT), MRI-PDFF for hepatic fat (retatrutide Phase 2 sub-analyses, [unverified primary source]), and treatment-emergent adverse event rates for GI burden

doi: 10.1210/jcem-64-5-896 37 BillingtonCJBartnessTJBriggsJLevineASMorleyJE

The zinc transporter SLC39A13/ZIP13 is required for connective tissue development