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The primary methods involved in the hyperaccumulation of toxic metals in plants include bio-activation of HMs in the rhizosphere through root microbe interfaces, enhanced activity of metal conveyor proteins in cell membranes, detoxification of metals by restricting them to apoplasts, chelation of HMs in the cytoplasm by multiple ligands, and sequestration of metals into the vacuole by multiple ligands (Kumar et al., 2019)