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In addition to being administered as a drug to cause oxidative stress, MTX has also been used to induce cachexia, anorexia, intestinal mucositis and digestive absorption problem in experimental animal models (Reference Boukhettala, Leblond and Claeyssens34Reference Sinno, Coquerel and Boukhettala36)

Thus, GSH depletion in vivo increases the hepatic MGO accumulation by impairing its GSH-dependent, GLO-mediated detoxification to d-lactate independent of oxidative stress [76]

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