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For the maximum LDH release control group, 4 l of lysis solution (Promega, USA) was added into each well and incubated for 30 mins to initiate cell lysis prior to the next step

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Clinical trials used doses of 0.3 4.5 mg/week, at a 2.5 mg/ml concentration, a 2.4 mg dose = 0.96 ml = 96 IU on the insulin syringe