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Materials and methods Cell lines and cell culture Small cell lung cancer cell line NCI-H69 was purchased from the American Type Culture Collection (Rockville, MD, USA) and maintained in RPMI-1640 (Cellgro, Herndon, VA, USA) supplemented with 10% (v/v) fetal calf serum (FCS), L-glutamate, sodium pyruvate, and HEPES buffer as described previously (Ma et al, 2003a)

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CRISPR screens in synthetic lethality and combinatorial therapies for cancer

The biological rationale is sound: vitamin D receptors are present in endometrial tissue, and a balanced vaginal and gut microbiome may reduce inflammatory interference with implantation

Blocking the HGF/c-Met signaling pathway can inhibit the damage of tumor cells to nerves and the vascularization of the tumor in nerves