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The cells were plated in DMEM containing 10% foetal bovine serum (FBS) and 5.5 mM glucose

It provides protection against the damaging effects of a wide range of noxious agents, such as alcohol and nonsteroidal anti-inflammatory drugs (NSAIDs), which are known to cause significant tissue injury

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Robust GLP-1 secretion by basic L-amino acids does not require the GPRC6A receptor

P2 was suspended in 10 vol of 0.32 M sucrose and 10 ml of the solution layered on a 0.8 and 1.2 M two-step discontinuous gradient, which was centrifuged at 61,000 x g for 2 h to separate the synaptosomes from the mitochondria (which forms the pellet)