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The RNAprotein complex was captured by streptavidin-couped Dynabeads (Thermo, 65606D) (room temperature, 30 min), washed with ice-cold 1 TENT buffer, mixed with 1 Laemmli sample buffer and heated for 10 min at 95 C for immunoblot analysis using anti-YBX1 antibody (ABclonal, A3534)

Apparently, the long-term effects of GPLC are related to the timed effects of different individual mechanisms

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In some embodiments, the amount of thermoreversible polymer (e.g., pluronic F127) in any formulation described herein is about 20% of the total weight of the formulation

Improves energy metabolism in cells, contributing to enhanced vitality