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The next 3 steps in mitochondrial -oxidation involve a hydration step, another oxidation step, and finally a hydrolytic reaction that requires CoA and releases acetyl-CoA and an acyl-CoA 2 carbon atoms shorter than the initial substrate
In this reaction, a suitable photosensitizer is excited by irradiation to an excited electronic state, and during deactivation, energy is transferred to surrounding oxygen molecules, where it forms singlet oxygen (Sies 1986)
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