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The working solution in the BCA protein concentration determination kit (P0012, Beyotime, China) was used to measure the absorbance of the sample at 562 wavelengths, and the sample concentration was calculated based on the standard curve

The abdomen offers the most convenient SC injection site for self-administration, with adequate subcutaneous fat and accessibility making the technique relatively straightforward after proper training

Preparation and reconstitution should be performed using sterile laboratory technique aligned with your protocol requirements