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10.1095/biolreprod.114.119032 16 HauserR.GaskinsA

The residual hematocrit was mixed immediately with 1 mL chilled, isotonic MBB (purchased from Calbiochem) buffer solution (pH 7.5) containing the following: 5 mmol MBB/L, 17.5 mmol Na 2 EDTA/L, 50 mmol potassium phosphate/L, 50 mmol serine/L, and 50 mmol boric acid/L