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All histopathological and immunohistochemical evaluations were performed by two independent observers blinded to the group assignments

Astrocytes are neural parenchymal cells that proliferate after injury to replace lost neural tissue, form borders, and protect and preserve neural parenchyma in different disorder contexts as discussed in multiple places in this article

Protocol Overview Duration: 28 days (1 vial) Application Schedule: Monday to Friday Daily application on weekdays only Weekend breaks are built into the protocol for balance and consistency Recommended Dose: Applied daily according to the established research protocol to ensure stable and controlled exposure over the 28-day cycle

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