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Genetic testing removes the trial-and-error mentality

Treatment starts at the lowest dose and is gradually increased every four weeks to help the body adapt and minimise side effects

After 2 days of expression, cells were harvested in ice-cold PBS and subsequently lysed in AP-MS buffer (10 mM Tris/Cl pH 7.5, 150 mM NaCl, 10% Glycerol, 0.5 mM EDTA, 0.5% Nonidet P40) supplemented with protease inhibitor cocktail (Roche, Rotkreuz, Switzerland) by sonification, followed by centrifugation (20,000 g for 10 min

Although FOXO4-DRI treatment did remove the senescent cells, the cartilage formation capacity of retained chondrocytes was not improved

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