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For the fasting and refeeding experiment, we used a modified version of the flow cytometry protocol with quadruplicate samples for the following conditions: (A) Day 1 fed, (B) Day 2 fed, (C) Day 2 fasted, (D) Day 3 fed, and (E) Day 3 refed

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Functionally, in the SLC30 family, ZnT1 functions primarily as a zinc exporter on the cell membrane, transporting cytoplasmic zinc ions across the membrane to the extracellular space, while other ZnT proteins are situated on the membranes of intracellular organelles