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Raw relative light unit (RLU) readings were expressed as the ratio of Venus to Nanoluc signals, and baseline-corrected BRET ratios normalised to vehicle readings to determine the agonist-induced effect

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Native CREB3L2 is a member of the CREB basic leucine zipper family of transcription factors and acts as a transducer of endoplasmic reticulum (ER) stress signals

Whether this level is sufficient to produce meaningful metabolic effects depends on individual receptor sensitivity

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