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If youve used this vial before, wipe the stopper on top with an alcohol swab

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To measure the intracellular level of GSH in yeast, YFP to CFP emission intensity ratio was recorded with 435 nm excitation filter and two emissions filters (485 nm for CFP and 535 nm for YFP) using LAS-AF software

Retrieved 14 February 2014

Because organosulfur compounds derived from alliinase-catalyzed reactions may play a role in some of the biological effects of garlic, some scientists recommend that crushed or chopped garlic be allowed to "stand" for at least 10 minutes prior to cooking (135)